Quantification of Cell Subpopulations, Fractions of Dead Cells and Debris in Cell Suspensions by Laser Diffractometry

Laser diffractometry was employed for size analysis in liver cell and blood cell suspensions to assess its suitability for characterizing cell populations. The method proved sensitive to detect subpopulations in liver cells (bimodal or trimodal distributions) and to quantify their volume fractions. Cell debris and aggregates of cells could also be quantified, dead cell populations recognized by their shift in the mean cell diameter. Laser diffractometry is therefore suitable for determining the quality of cell isolations (e.g. by liver perfusion) or for following alterations in cell populations during culture of cells in suspension. Analysis of human blood allowed differencial ions to be made between thrombocytes and other blood cells. No peak separation was obtained for the populations of erythrocytes and granulocytes due to their similarity in size. Monocytes could not be detected due to their extremely low number in the blood indicating the limit of the method.